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For example, sorbitol can be used as a replacement sugar in MacConkey agar to assist in the isolation and differentiation of enteropathogenic E coli serotypes such as E coli O157H7 by the presence of white circular colonies that are nonsorbitol fermenting Preparation of MacConkey AgarSuccessful E coli transformations create colored colonies on some media For example, growth on a MacConkey agar plate shows that E coli are not inhibited by bile salts and crystal violet The pink color of the bacterial growth indicates E coli can ferment lactose and tells you that it is a gramnegative bacteriumNutrient Agar, a less nutritious medium (right) 327 INFLUENCE OF AGE ON PIGMENT PRODUCTION A Serratia marcescens grown on Sheep Blood Agar after 24 hours B The same plate of S marcescens after 48 hours Staphylococcus Epidermidis An Overview Sciencedirect Topics What color is e coli on nutrient agar